Fig. 5.

Esophageal cancer cells induce CD39 expression on human NK cells via secretion of IL-6. a PBMC from healthy donors were cultured with recombinant human IL-6 for 48 h. b Anti-IL-6 receptor antibody was added into the culture system with PBMC and IL-6. c and d PBMC were cultured with conditioned media (CM) from TE-1 and TE-7 cells in the presence or absence of anti-IL-6 receptor antibody for 48 h. The surface CD39 expression on NK cells was analyzed by flow cytometry. Representatives of three independent experiments. *p < 0.05; **p < 0.01