Fig. 7.

Effects of the combination therapy of apatinib and PD-1 mAb on tumor immunomicroenvironment. The decreased ratio of Treg cell subsets in T lymphocytes of CT26 and 4T1 tumors (a and c). The variety of M2-like macrophages (b and d) and myeloidderived suppressor cells (e) in immune cells in CT26 and 4T1 tumors, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. f The heat map displayed the distinct expression of the genes associated with immune function in CT26 and 4T1, using MCP-counter scores. -VEGFA: the group of mice bearing VEGFA-overexpressed tumor cells, -NC: the group of mice bearing tumor cells with empty vector