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Frequency of IFNγ-specific CD8 + splenocytes 14 days after vaccination of 14–18-week-old tumor-bearing TRAMP mice with prostate tumor antigen-VLP vaccines. Splenocytes from vaccinated mice (n = 7) were tested by intracellular cytokine stain, as described in the legend to Fig. 2a. All antigen-specific response were significantly higher than that of background unstimulated cultures (t test, P < 0.05)
