Fig. 1.

Expression of PD-1 and TIM-3 by CD160⁺ vs. CD160⁻ CD8⁺ T cells. Fresh circulating CD8⁺ T cells were stained directly ex vivo. a Representative gating of CD160 vs. PD-1 and CD160 vs. TIM-3 in one representative individual, pre-gated on CD8⁺ T cells. b Frequencies of PD-1⁺CD160⁻ and PD-1⁺CD160⁺ cells in total CD8⁺ T cells, and TIM-3⁺CD160⁻ and TIM-3⁺CD160⁺ cells in total CD8⁺ T cells. c Percent of CD160⁻ or CD160⁺ CD8⁺ T cells that expressed PD-1 and TIM-3. d Geometric mead fluorescence intensity (GMFI) of PD-1 and TIM-3 in PD-1⁺ and TIM-3⁺ cells, respectively, from CD160⁻ or CD160⁺ CD8⁺ T cells. ns not significant. ***p < 0.001