Table 2.
Changes of lymphocyte subsets during chemotherapy
| Total population | ||||||
|---|---|---|---|---|---|---|
| Before chemotherapy | After chemotherapy | P value | Control group | P-value (patients vs. control) | ||
| (a) B lymphocytes | ||||||
| B Lymphocytes | 0.076 [0.039; 0.160] | 0.049 [0.024; 0.106] | 0.001 | 0.230 [0.173; 0.270] | 0.000 | |
| Naive | 0.049 [0.021; 0.111] | 0.025 [0.013; 0.056] | 0.001 | 0.115 [0.087; 0.189] | 0.002 | |
| Memory | 0.003 [0.002; 0.008] | 0.002 [0.000; 0.004] | 0.001 | 0.005 [0.004; 0.065] | n.s. | |
| Class switch | 0.011 [0.006; 0.020] | 0.006 [0.003; 0.012] | 0.011 | 0.030 [0.023; 0.042] | 0.001 | |
| Transitory | 0.002 [0.000; 0.005] | 0.000 [0.000; 0.002] | n.s. | 0.006 [0.003; 0.006] | 0.005 | |
| (b) CD8 + T lymphocytes | ||||||
| CD8 + Lymphocytes | 0.214 [0.122; 0.374] | 0.233 [0.120; 0.319] | n.s. | 0.275 [0.212; 0.415] | n.s. | |
| CD4 + CD8+ | 0.005 [0.002; 0.013] | 0.003 [0.002; 0.012] | n.s. | 0.006 [0.001; 0.009] | n.s. | |
| Memory | 0.059 [0.040; 0.126] | 0.086 [0.034; 0.128] | n.s. | 0.101 [0.051; 0.137] | n.s. | |
| Central memory | 0.028 [0.015; 0.062] | 0.025 [0.016; 0.048] | n.s. | 0.004 [0.002; 0.014] | 0.000 | |
| Naive | 0.043 [0.017; 0.091] | 0.034 [0.016; 0.066] | n.s. | 0.005 [0.002; 0.015] | 0.001 | |
| Effector memory | 0.054 [0.037; 0.110] | 0.067 [0.030; 0.119] | n.s. | 0.121 [0.074; 0.172] | 0.007 | |
| EMRA | 0.050 [0.023; 0.110] | 0.068 [0.031; 0.120] | n.s. | 0.140 [0.106; 0.187] | 0.003 | |
| Early | 0.082 [0.048; 0.168] | 0.078 [0.052; 0.174] | n.s. | 0.176 [0.087; 0.262] | n.s. | |
| Intermediate | 0.010 [0.006; 0.185] | 0.014 [0.008; 0.030] | n.s. | 0.026 [0.013; 0.045] | 0.001 | |
| Late | 0.044 [0.024; 0.117] | 0.054 [0.024; 0.126] | n.s. | 0.081 [0.033; 0.151] | n.s. | |
| Exhausted | 0.055 [0.034; 0.107] | 0.068 [0.036; 0.118] | n.s. | 0.140 [0.078; 0.266] | 0.006 | |
| Terminal effector | 0.033 [0.021; 0.114] | 0.037 [0.015; 0.101] | n.s. | 0.026 [0.013; 0.092] | n.s. | |
| HLA-DR+ | 0.066 [0.031; 0.131] | 0.076 [0.032; 0.153] | n.s. | 0.007 [0.003; 0.025] | 0.000 | |
| CD69+ | 0.037 [0.018; 0.065] | 0.043 [0.022; 0.773] | n.s. | 0.054 [0.036; 0.070] | n.s. | |
| Regulatory | 0.000 [0.000; 0.002] | 0.001 [0.000; 0.002] | n.s. | 0.000 [0.000; 0.001] | n.s. | |
| CD25+ | 0.001 [0.000; 0.001] | 0.001 [0.000; 0.001] | n.s. | 0.000 [0.000; 0.001] | n.s. | |
| (c) CD4 + T lymphocytes | ||||||
| CD4 + Lymphocytes | 0.587 [0.367; 0.802] | 0.443 [0.236; 0.679] | n.s. | 0.595 [0.442; 1.128] | n.s. | |
| Memory | 0.317 [0.226; 0.547] | 0.301 [0.157; 0.376] | n.s. | 0.333 [0.258; 0.512] | n.s. | |
| Central memory | 0.220 [0.141; 0.331] | 0.178 [0.103; 0.270] | n.s. | 0.079 [0.061; 0.193] | 0.007 | |
| Naive | 0.193 [0.065; 0.330] | 0.104 [0.035; 0.272] | n.s. | 0.216 [0.106; 0.365] | n.s. | |
| Effector memory | 0.119 [0.068; 0.203] | 0.099 [0.068; 0.198] | n.s. | 0.273 [0.200; 0.385] | 0.010 | |
| EMRA | 0.008 [0.002; 0.020] | 0.005 [0.002; 0.016] | n.s. | 0.050 [0.036; 0.088] | 0.001 | |
| HLA-DR+ | 0.058 [0.043; 0.082] | 0.046 [0.037; 0.085] | n.s. | 0.018 [0.008; 0.030] | 0.001 | |
| CD69+ | 0.049 [0.027; 0.117] | 0.035 [0.021; 0.071] | n.s. | 0.034 [0.020; 0.039] | n.s. | |
| Th1 | 0.042 [0.018; 0.069] | 0.030 [0.012; 0.060] | n.s. | 0.025 [0.011; 0.063] | n.s. | |
| Th2 | 0.049 [0.025; 0.091] | 0.042 [0.023; 0.076] | n.s. | 0.040 [0.023; 0.071] | n.s. | |
| Th17 | 0.033 [0.020; 0.054] | 0.031 [0.021; 0.050] | n.s. | 0.049 [0.034; 0.071] | n.s. | |
| Regulatory | 0.027 [0.015; 0.047] | 0.012 [0.010; 0.033] | n.s. | 0.017 [0.011; 0.026] | n.s. | |
| CD25+ | 0.008 [0.005; 0.014] | 0.009 [0.004; 0.014] | n.s. | 0.003 [0.001; 0.003] | 0.000 | |
| CD4/CD8 | 2.403 [1.685; 3.842] | 1.824 [1.328; 3.482] | n.s. | 2.276 [1.641; 3.390] | n.s. | |
| (d) NK cells | ||||||
| NK Cells | 0.131 [0.093; 0.240] | 0.131 [0.073; 0.232] | n.s. | 0.240 [0.153; 0.463] | n.s. | |
| CD56 + CD16+ | 0.036 [0.017; 0.083] | 0.043 [0.012; 0.072] | n.s. | 0.160 [0.108; 0.255] | 0.000 | |
| CD56bright CD16dim | 0.003 [0.001; 0.007] | 0.003 [0.001; 0.006] | n.s. | 0.014 [0.008; 0.027] | 0.000 | |
| CD56dim CD16bright | 0.004 [0.002; 0.018] | 0.006 [0.002; 0.010] | n.s. | 0.019 [0.015; 0.026] | 0.020 | |
| NK/T | 0.046 [0.025; 0.098] | 0.053 [0.021; 0.083] | n.s. | 0.005 [0.020; 0.135] | n.s. | |
(a) B lymphocytes (b) CD8 + T lymphocytes (c) CD4 + T lymphocytes (d) NK cells. Numbers are median values/ml and interquartile ranges in square brackets. P < 0.022 = significant
n.s not significant, EMRA effector memory RA+