Fig. 1.
Immunization with the MAG-Tn3 vaccine induces activation of a specific anti-TT T-cell response. Patients were immunized on days 0, 21, 42, 63, 84, and 105 with the MAG-Tn3 vaccine formulated with the immunostimulant AS15. Patients 01, 02, and 03 received intramuscular injections of 30 µg MAG-Tn3, whereas patients 04, 05, 06, and 07 received 100 µg MAG-Tn3. PMBCs were harvested at various times after immunization and stimulated for 18 h with either medium or the tetanus toxin (TT830–844) peptide. IFNγ production was evaluated by ELISPOT and is expressed as the mean of spot forming colonies (SFC) per 106 cells ± SEM of quadruplicates from two independent experiments. The statistical significance of the differences was determined by the unpaired Student’s t test, comparing the SFC obtained with medium or TT peptide for each timepoint. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001