Fig. 3.

LincRNA-p21 down-regulated TAMs promoted tumor cells apoptosis, inhibited their migration and invasion. a, e LincRNA-p21 down-regulated TAMs promoted tumor cell apoptosis. 4T1 and LLC were co-cultured with lincRNA-p21 down-regulated macrophages for 48 h, respectively. The tumor cells were collected and stained with Annexin-V and 7AAD to detect the apoptosis using flow cytometry, respectively. b LincRNA-p21 down-regulated TAMs inhibited 4T1 cell proliferation. The proliferation of 4T1 cells co-cultured with lincRNA-p21 down-regulated macrophages was measured using Cell Counting Kit-8 (CCK-8) at indicated point. c, f LincRNA-p21 down-regulated TAMs inhibited tumor cells migration. Transwell assays were performed to detect migration ability of tumor cells (4T1 and LLC) co-cultured with lincRNA-p21 down-regulated TAMs for 24 h. The left panels show representative images and right panels show statistical analysis. d LincRNA-p21 down-regulated TAMs inhibited 4T1 cells invasion. Wound-healing assay was used to detect the invasive ability of 4T1 cells co-cultured with lincRNA-p21 down-regulated TAMs. g LincRNA-p21 knockdown could not increase ROS production in TAMs. The left panels show representative images and right panels show statistical analysis. h The expression of Fas on 4T1 cell and FasL on TAMs was assessed by flow cytometry. i R7050 (10 nM) was used to pretreat 4T1 for 6 h and after that 4T1 was co-cultured with lincRNA-p21 down-regulated macrophages for 48 h; 4T1 cells were collected and stained with Annexin V and PI to detect apoptosis. All the data were obtained from three independent experiments and the representative images are shown. *P<0.05 relative to control. N.S. means no significance. LLC and SLP1 mean Lewis lung carcinoma cells and lincRNA-p21 downregulated macrophages