Figure 4. CARG-2020-induced immune memory is transferable.
A. Diagram of spleen transfer study design. mCherry+ TKO mouse ovarian cancer cells were injected i.p. in C57BL/6 mice. CARG-2020 donor mice were treated with CARG-2020 (3 doses of 1×106 PFU CARG-2020) when mCherry ROI fluorescence reached 3.2×108 PFU/sec. Splenocytes were isolated from CARG-2020 donor mice 35 days after the start of treatment when mice were disease-free. Splenocytes were injected i.p. in another set of TKO-bearing mice in Dormant phase of the disease. Control recipients received splenocytes isolated from non-treated tumor-bearing donor mice. Non-treated tumor-bearing donor mice received PBS and spleens were isolated when mCherry ROI area reached 1×109 photons/sec; B. Representative images of Recipient mice showing mCherry+ i.p. tumor burden. C. overall survival was defined as day mCherry ROI area reached 1×109 PFU/sec or abdominal width reached 3.4 cm and calculated using Kaplan Meier analysis.