Figure 3.
Antibody formats most frequently used in research assays: IgM, Immunoglobulin M; IgG, Immunoglobulin G; F(ab’)2, divalent fragment antigen binding; F(ab), fragment antigen binding, now usually named Fab; scFab, single chain fragment antigen binding; scFv-Fc, single chain fragment variable::Fc fusion protein; scFv, single chain fragment variable; diabody, dimerized scFv; and single domain antibodies/nanobody. Constant regions are colored dark blue (γ chains), green (µ chains), or light blue (κ or λ chains); variable (antigen binding) regions in pink. Yellow lines indicate disulfide bonds, red lines peptide linker, purple lines peptide tags. C, constant region, V, variable region, HC, heavy chain; LC light chain. Elements are not drawn to scale.