Fig. 1.

MDSCs from tumor-bearing mice bind AFP at significantly higher levels than non-MDSCs. Splenocytes were obtained from CBA mice bearing Ehrlich solid carcinoma 3 weeks after tumor inoculation. G-MDSCs (Gr-1^highLy-6G⁺), M-MDSCs (Gr-1^dimLy-6G^–), and non-MDSCs were isolated by immunomagnetic separation, incubated with 100 µg/ml AFP-FITC and assessed for AFP⁺ events by flow cytometry (a, c). Mononuclear fraction isolated from tumor-bearing mice was labeled with anti-Gr-1, anti-CD11b, AFP-FITC, and the frequency of AFP⁺ events was analyzed in G-MDSC (Gr-1^highCD11b⁺), M-MDSC (Gr1^dimCD11b⁺), monocyte (Gr1⁻CD11b⁺), and non-MDSC (Gr1⁻CD11b⁻) gates using flow cytometry (b, d). Each group included 7 mice. Representative flow cytometry and cumulative results for each group are indicated. Student’s t test showed that there was a significant difference between the groups, as indicated: *p < 0.05