Fig. 1.

TRPM2 affects CXCL2 expression and neutrophil chemoattraction. a Representative IHC staining of Ly6G⁺ neutrophils (red, arrows) in 4T1 TRPM2^−/− and TRPM^+/+ tumors (n = 5). b Number of neutrophils per 4T1 TRPM2^−/− and TRPM^+/+ tumors area. c Relative expression of TRPM2 in control and TRPM2kd 4T1 cells. d Relative expression of CXCL1, CXCL2 and CXCL5 in control and TRPM2kd 4T1 cells. e CXCL2 secretion by control and TRPM2kd cells. Relative expression of TRPM2 (f) and CXCL2 (g) in control and TRPM2kd AT3 cells. Relative expression of TRPM2 (h) and CXCL2 (i) in control and TRPM2kd LLC cells. j Neutrophil migration towards vehicle and media conditioned by control or TRPM2kd cells. k Neutrophil migration in the absence (Cont.) or presence of CXCL2 neutralizing antibody (αCXCL2). l Relative expression of CXCL2 in tumor cells selected with H₂O₂. m Relative expression of CXCL2 in control, H₂O₂ treated and H₂O₂ + BAPTA-AM treated 4T1 cells. These experiments were repeated at least three times with similar results. Statistical significance was determined using unpaired student’s t test. *p < 0.05, **p < 0.01