Fig. 2.

CXCL2 overexpression in TRPM2kd 4T1 cells restores neutrophil chemoattraction. a Migration plots of five random neutrophils towards control (left), TRPM2kd (middle), and CXCL2-overexpressing TRPM2kd 4T1 cells (right). b Cumulative distance neutrophils travel when co-cultured with control, TRPM2kd or CXCL2 overexpressing TRPM2kd 4T1 cells (n = 10). c Absolute distance neutrophils migrated from their initial position when cultured with control, TRPM2kd, or CXCL2 overexpressing TRPM2kd 4T1 cells (n = 10). d Localization of neutrophils (smaller cells, yellow arrows) when cultured with control, TRPM2kd, or CXCL2 overexpressing TRPM2kd cells (larger cells, red arrows) at time 0 and at 2 h. e Relative expression of CXCL2 in control, TRPM2kd, and CXCL2 overexpressing TRPM2kd (TRPM2kd–CXCL2) 4T1 cells. f Relative expression of TRPM2 in control and CXCL2 overexpressing TRPM2kd 4T1 cells. g Neutrophil chemotaxis towards media conditioned by control, TRPM2kd and CXCL2 overexpressing TRPM2kd 4T1 cells. Results represent the average of ten representative cells. These experiments were repeated three times with similar results. Statistical significance was determined using unpaired student’s t test. *p < 0.05, **p < 0.01