Fig. 3.

Higher M2/M1 ratio is associated with glioma progression. Vertical scatter plots signify the gene expression at mRNA level by RT-qPCR in low- and high-grade gliomas and normal brain tissues for the a CD163; b CCL3; c IL-10; d TGF-β. e Ratio of CD163/CCL3 is shown in gliomas and normal brain tissues. Each dot represents a single individual. Data expressed as arbitrary units after normalization are designated as mean ± SEM. NBC: normal brain control, LGG: low-grade glioma, HGG: high-grade glioma. *p < 0.05; **p < 0.01; ***p < 0.001. N represents number of individuals. Survival correlations of M1 and M2c macrophage markers in glioma patients; OncoLnc tool was used to explore the survival correlations of f CCL3 and CD163; g IL-10 and TGFβ1 in glioma patient data in TCGA portal. Kaplan–Meier plots show the survival correlation in glioma patients expressing the above genes, and the level of significance is represented by the log rank p value (< 0.05). Red and blue lines indicate the cohort of glioma patients with high and low expression of the subject genes, respectively