Fig. 3.

ST2-expressing Tregs are enriched in the HNSCC TME. a Gating strategy for CD3⁺CD4⁺ cells. b Proportions of ST2-expressing Tregs among healthy donor PBMCs, (PBMC-HD), PBMCs from HNSCC patients (PBMC-HNSCC), and TILs. c Quantitative analysis of the percentage of ST2⁺Foxp3⁺ Tregs among total Tregs. d Proportion of ST2-expressing Tregs in co-cultures (DCs and CD4⁺ T cells) containing control DCs (control DC) or SNU899-stimulated DCs (SNU899 DC). Data were analyzed by one-way ANOVA. e Quantitative analysis of the percentage of ST2⁺Foxp3⁺ Tregs among total Tregs in co-cultures of control DCs or SNU899-stimulated DCs. The Student’s t test was used for data analysis. Data are the mean ± SEM and representative of three independent experiments. n.s. not significant, ***P < 0.001