Fig. 4.

MiR-935 regulates INT-HA₅₀-induced M2-polarized THP-1 macrophages. a Schematics of the approach. i). THP-1 derived macrophages were transfected with a negative control (NC), miR-935 inhibitors (40 nM) or mimics (20 nM) for 48 h. ii). THP-1-derived macrophages were treated with INT-HA₅₀ for 24 h; then, the cells were transduced with NC and mimics for 48 h. b Gene expression levels of Arg1, CCL18 and IL10 in macrophages as determined by RT-qPCR. The representative histograms and quantification of the fold changes are shown (mean ± SD, n = 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). c Cytokine levels of CCL18 and IL10 in the media from macrophages (mean ± SD, n = 3 independent experiments. *p < 0.05)