Fig. 5.

b-AP15-induced upregulation of NKG2DL enhances NKG2D-mediated NK cell lysis. a Indicated MCL cells were exposed to the indicated concentration of b-AP15 for 24 h or were left untreated and subsequently employed in ⁵¹chromium release cytotoxicity assays with polyclonal NK cells. Data points represent mean of three technical replications; bars represent SD. Student’s t test was used to determine statistical significance between control and treated cells (*p < 0.05). One representative result of at least three independent experiments with similar results is shown. b MCL cells were exposed to the indicated concentration of b-AP15 for 24 h or were left untreated and subsequently employed in ⁵¹chromium release cytotoxicity assays with polyclonal NK cells in the presence or the absence of a blocking NKG2D antibody (6H7) or the respective isotype control. Data are presented as mean of three technical replicates; bars represent SD. Student’s t test was used to determine statistical significance of differences (*p < 0.05). One representative result from at least three independent experiments with similar results is shown