Fig. 5.

FcγRIIa and FcγRIIIa involved with phagocytosis of tumors. a Expression of FcγRIIa and FcγRIIIa from thawed PBMC and those cultured overnight on adherent and non-adherent tissue culture flasks. b Phagocytosis was evaluated as described in Fig. 1. Comparison of phagocytosis from PBMC cultured in adherent and non-adherent tissue culture flasks. All samples were performed in triplicate; means ± SEM and regression line are plotted on the graph. Two-way ANOVA with Sidak’s multiple comparisons test was used to determine the significance. Asterisk represents significance between adherent and non-adherent. Results are representative of two independent experiments. c Phagocytosis was evaluated as described in Fig. 1 with a single concentration of trastuzumab (200 ng/ml). FcγRIIa and FcγRIIIa Fab were used at a concentration of 10 µg/ml. All samples were performed in triplicate; means ± SEM are plotted on the graph. One-way ANOVA with Tukey’s multiple comparisons test was used to determine significance. Asterisk represents significance between untreated and isotype controls and blocking antibodies. ^†Represents significance between blocking of FcγRIIa and FcγRIIIa or FcγRIIa + FcγRIIIa. Results are representative of two independent experiments