FIG. 3.
Primary transcription (A), amplified gene expression (B), accumulation of gene products (C), and genome replication (D) during 9301B and 9301V virus infection in B95a cells. GAPDH expression was used as an internal control in the experiments in panels A and B. (A and D) PCR products; (B) Northern blot; (C) Western blot. RNAs extracted from infected cells at various times p.i. were directly reverse transcribed and PCR amplified with specific primers (A, top, and D), or the RNAs at 4 h p.i. were diluted to 10−1, 10−2, and 10−3 before being subjected to reverse transcription-PCR (A, bottom).