TABLE 1.
Comparisons of 9301B and 9301V for their growth in Vero cells and pathogenicity for cynomolgus monkeysa
| Characteristic | 9301Bb | 9301Vb |
|---|---|---|
| Growth in Vero cells (TCID50/ml) | <10 | 1.7 × 106 |
| Symptom and histopathology in monkeys | ||
| Rash | ++ | − |
| Leukopenia | ++ | − |
| Body wt loss | ++ | − |
| Syncytium formation in lymphoid organsc | ++ | + |
a
Infections of Vero cells with 9301B and 9301V viruses were initiated at an input MOI of 0.2 TCID50/cell, and the titers on day 3 were assayed by measurement of TCID50 in B95a cells. Infections of monkeys were initiated with 105.5 TCID50 of the respective viruses, and symptoms and pathological changes were observed as described previously (13).
b
The degree of phenotype expression is shown by ++ (extensive), + (moderate), and − (not apparent).
c
Lymphoid organs include the spleen, thymus, and lymph nodes.