Fig. 1.

Construction and transient expression of HRG1β-CAR in HEK-293T cells. a Lentiviral vector construct of HRG1β-CAR. SP signal peptide, ECD extracellular domain, TM transmembrane portion, ICP intracellular portion. b Agarose gel electrophoresis of PCR and restricted DNA products. Left M, 1 kb DNA marker, right M, DL5000 DNA marker, lane 1, two restricted DNA products: pLenti6.3/V5-DEST plasmid (9387 bp) and HRG1β-CAR gene(1312 bp), lane 3, two restricted DNA products: pENTR3C plasmid (2723 bp) and HRG1β-CAR gene(1312 bp), Lane 2 and 4, PCR product: HRG1β-CAR gene (1312 bp). c HRG1 was detected by flow cytometry in HEK-293T cells transduced with HRG1β-CAR. d CD3ζ was detected by western blotting in HEK-293T cells transduced with HRG1β-CAR. Data are representative images of 3 independent experiments