Fig. 4.

HER3 knockdown confers resistance of breast cancer cells to cytolysis by HRG1β-CAR-T cells. a–c SK-BR-3 cells were transfected with indicated siRNAs, and subject to qRT-PCR (a), western blotting (b) and flow cytometry (c) analysis. d Lysis of target cells by control T or HRG1β-CAR-T cells after 4-h incubation at increasing E:T ratios. Data are represented as the mean ± SEM of n = 3 replicates or representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test)