Fig. 4.

Selective response of TCR-engineered T-cells against cells expressing the L265P mutation. a Peripheral CD8⁺ T cells isolated from healthy donors and virally transduced to express L265P-specific T-cell receptors (TCRs) were co-cultured with K562 target cells expressing different combinations of WT or mutant MYD88 and human HLA-B*07. Experiments included also cells pulsed with a peptide encompassing the mutation. IFNγ release was analyzed by ELISA. b K562 cells transduced with mutant MYD88 and HLA-B*07 are specifically killed by TCR-engineered T cells. The proportion of apoptotic target cells is measured by flow cytometry via intracellular staining of activated caspase-3