Fig. 1.

DC matured with a cocktail consisting of OK432, CL097, and PGE₂ express high amounts of co-stimulatory molecules and CCR7. HLA-DR was highly expressed on all cell populations. CD83 was increased in all matured DC populations. CCR7 was significantly increased (p < 0.05) in every group matured with PGE₂ compared to iDC. CD80 was significantly higher expressed in ccDC and DC treated with OK432, CL097, and PGE₂ (p < 0.05) compared to iDC, while the increase of CD86 expression did not reach statistical significance. IDC immature DC, ccDC: DC matured with Jonuleit cytokine cocktail TNF, IL-6, IL-1β, and PGE₂; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097 and 0.5 µg/ml PGE₂; MFI: median fluorescence intensity. Each symbol represents a different donor