Fig. 3.

OK432-stimulated DC secrete more cytokines relevant for Th1 cell stimulation. All OK432-matured DC had a significant increase in IL-12p70 secretion compared to ccDC and iDC that was further elevated with the addition of CL097, but decreased by PGE₂. The amount of IL-12p70 was reduced by the addition of PGE₂, but the cells still produced significantly more IL-12p70 than ccDC (p < 0.05). This pattern was also observed with RANTES/CCL5, MIP-1α/CCL3 and MIP-1β /CCL4. IL-12p40 was highly elevated by the combination of OK432, CL097 and PGE₂, albeit not statistically significant compared to other matured DC groups. IDC: immature DC; ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE₂; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097, and 0.5 µg/ml PGE₂. Each symbol represents a different donor