Fig. 4.

Increased IL-12p70 secretion by DC upon addition of TLR ligand containing maturation cocktails. 3-day DC were matured with different maturation cocktails containing TLR ligands: (i) Kalinski cocktail [35]: TNF, IL-1β, IFN-α, IFN-γ, and polyI:C; (ii) Lövgren cocktail [36]: TNF, IFN-γ, R848, and polyI:C; (iii) Zobywalski cocktail [37]: TNF, IL-1β, IFN-γ, PGE₂, R848, and polyI:C. IDC: immature DC; ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE₂; OK432: DC matured with 0.1 KE/ml OK432; OK432 + CL097: DC matured with 0.1 KE/ml OK432 and CL097; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097, and 0.5 µg/ml PGE₂. Each symbol represents a different donor