Fig. 6.

OK432-matured DC are able to induce antigen-specific T cells. Autologous NAC (PBMC depleted of monocytes) were co-cultured with PPD-loaded DC matured with noted stimuli for 7 days, and antigen-specific IFN-gamma secretion by CD4 + and CD8 + cells was analyzed by flow cytometry with ccDC as stimulators (+/− PPD). In some experiments (donors 12–14), the induced NAC were rested for 5 days prior to the IFN-g secretion assay. ccDC: DC matured with Jonuleit cytokine cocktail TNFα, IL-6, IL-1β, and PGE₂; OK432 + CL097 + PGE2: DC matured with 0.1 KE/ml OK432, CL097, and 0.5 µg/ml PGE₂. Each symbol represents a different donor