Fig. 3.

Repolarisation of M2 macrophage enhances the phagocytosis of macrophages and increases sensitivity of human laryngeal cancer cells to cisplatin (CDDP). a Schematic representation of phagocytosis assay. GFP-labelled Hep-2 cells (Hep-2-GFP) were co-cultured with pre-treated M2 macrophage. The macrophage phagocytosis of tumour cells was examined 24 h post-incubation. b Flow cytometry demonstrating the signal intensity of GFP in the F4/80 positive macrophage. c Quantitative analysis of GFP positive macrophage after incubation. ***p < 0.001 (versus vehicle-treated group). d Schematic representation of drug sensitivity assay. The M2 macrophages were pre-treated with vehicle or CQ (20 μM) for 24 h; the conditioned medium (CM) was collected. Hep-2 cells were incubated with conditioned medium (CM) and then treated with cisplatin (CDDP, 2.5 μM) for 48 h. Cell apoptosis was examined by annexin V/propidium iodide apoptosis assay. e Flow cytometry demonstrating the apoptotic cells. f Quantitative analysis of apoptotic cells. **p < 0.005. ns no significant difference