Table 2.

Characterization of lung TIL pre- and post-REP

Pt.	Isolat meth.	REP day 0 (pre-REP)			REP day 14 (post-REP)
		CD8+ CD3+ (%)	CD4+ CD3+ (%)	CD56+ CD3− (%)	Fold exp.	Cell no. × 109	Funct.b	CD8+ CD3+ (%)	CD4+ CD3+ (%)	CD28+ CD8+ (%)	CD28+ CD4+ (%)	41BB+ CD8+ (%)	41BB+ CD4+ (%)	PD1+ CD8+ (%)	PD1+ CD4+ (%)
1	Digest	24	69	6	1746	79	11,209	42	58	8	48	2	6	22	28
	Frag. 5	5	86	9	1235	56	12,980	2	98	0	81	0	10	2	85
	TRCa	9	80	12	558	25	561	14	86	3	36	1	17	5	36
2	Digest	37	40	23	1596	72	22,208	83	17	16	12	3	2	44	11
	Frag. 5	51	43	6	1546	70	4282	79	21	9	11	3	2	28	18
	TRC	35	58	8	1495	67	1568	35	65	4	36	1	6	22	56
3	Digest	36	61	3	1107	50	15,836	20	80	2	49	1	6	7	34
	Frag. 3a	86	12	2	969	44	1642	96	4	10	3	3	0	12	2
	TRC	50	49	2	631	28	1211	58	42	9	19	2	3	30	19
4	Digesta	31	57	12	983	44	30,445	62	38	10	28	2	3	41	23
	Frag. 4	14	78	7	1204	54	2603	40	60	7	45	1	4	28	49
	TRC	30	61	9	925	42	12,603	57	43	10	34	2	3	33	26
5	Digest	37	63	27	892	40	26,844	3	97	0	16	0	4	2	77
	Frag. 2	23	71	6	923	42	12,261	6	94	0	24	0	2	4	79
	TRC	15	70	16	1000	45	4021	67	33	7	10	7	1	13	23
Av.		32	60	10	1121	50	10,685	44	56	6	31	2	5	19	38
± SD		± 20	± 19	± 7	± 349	± 16	± 9748	± 30	± 30	± 5	± 20	± 2	± 4	± 14	± 25

Isolat. meth. isolation method, exp expansion, sti stimulation, ca autologous carcinoma

^aFull-scale rapid expansion procedure, including microbiological testing

^bTIL functionality was determined as IFNγ secretion (pg/ml) following overnight incubation with the anti-CD3 antibody OKT-3