Fig. 2.

Binding of basal and hyperimmune sera from vaccinated patients to NeuGcGM3-expressing X63 cells. Serum samples from vaccinated patients were incubated with target cells and analyzed by flow cytometry. a Binding to X63 cells are expressed as percentage of MFI obtained wit basal samples for each patient. Kruskal–Wallis followed by Dunnett’s multiple comparison test was used to analyze differences between groups; p < 0.001 basal vs month 3. b, c Direct correlation exhibited between MFI, depicted as percentage of basal samples, and anti-NeuGcGM3 IgG titer (b) or and anti-NeuGcGM3 IgM titer (c). Pearson r test was used to analyze the results; p < 0.01, r = 0.4339, MFI vs log IgG titer; r = 0.4454, MFI vs log IgM titer