Fig. 2.

DC inhibitory effects of imatinib and nilotinib last for 3 days. Peripheral blood adhering monocytes cultured with GM-CSF and IL-4 were exposed to imatinib or nilotinib (1 and 3 µM) for 7 days with addition of LPS as maturation stimulus on day 6. Subsequently, cells were cultivated for 3 days in fresh medium before harvest. The phenotype of the recultivated DCs was analyzed by FACS and compared to the vehicle control DMSO (white column). Results of pooled data of at least 16 donors are shown. Together, these data show long-lasting effects of TKI treatment on DC activation