Fig. 1.

A2aR blockade with CPI-444 modulates checkpoint expression and enhances acute immune response to vaccine. C57BL/6 mice immunized with vaccinia-OVA (10⁶ PFU) by retro-orbital (r.o.) injection received either vehicle or CPI-444 (10 mg/kg) p.o. daily for 5 days starting 1 day post-infection. a Flow cytometry showing time course of Tet-OVA+ T cell response monitored by cheek bleeding using tetramer-OVA staining. b Data summary plot for Tet-OVA+ T cell response on day 7 post-infection. c–e Flow cytometric analyses of PD-1 (c), TIM-3 (d), and LAG-3 (e) expression on Tet-OVA+ T cells harvested on day 7 (PD-1) and day 11 (TIM-3, LAG-3) are shown as representative MFI histograms (left) and summation data plots (right). f–g Data summary plots from flow cytometry analyses of PD-1, TIM-3, LAG-3 (f) expression, and FoxP3 (g) expression on Tregs in the peripheral blood on day 11 post-infection. Error bars represent SEM. Data are representative of three independent experiments with n = 4–5 per group. *p < 0.05, **p < 0.01 using two-tailed Student’s t test