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. 2019 Feb 19;68(5):709–720. doi: 10.1007/s00262-018-02292-7

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© The Author(s) 2019

OpenAccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Tyrosinase-triggered IFN-γ spots are produced primarily by CD8 cells. PBMC from HD #36 were tested “unfractionated”, or after depletion of CD4 (CD4⁻) or CD8 (CD8⁻) cells via magnetic beads. These cells were then cultured either with tyrosinase (Tyr), CEF peptides (CEFpp32), CPI, or left unstimulated (media) and IFN-γ was detected after 24 h. a The original wells are shown. b The percentage reduction in spot forming unit (SFU) counts is shown after CD4 or CD8 cell depletion, representing the response of unfractionated PBMC as 100%