Fig. 3.

Characterization of aAPCs. a Size distribution and zeta potential distribution of aAPC^{IL-2+anti-CTLA4}. b Phenotype analyses of aAPC, aAPC^IL-2, aAPC^anti-CTLA4, and aAPC^{IL-2+anti-CTLA4}. All types of aAPCs were stained with PE-anti-H-2K^b and FITC-anti-hamster IgG to detect the co-immobilization of H-2K^b/TRP2-Ig dimers and anti-CD28 mAbs onto PLGA-MPs. Blank PLGA-MPs that were pre-blocked with 30% BSA displayed baseline staining. c, d Release profiles of IL-2 and anti-CTLA-4 from the four types of aAPCs over the course of 28 days, as detected by ELISA kits