Fig. 1.

WT1 protein expression on B. longum 420. a Schematic drawing of recombinant B. longum 420. The recombinant WT1 protein including one CD4⁺T and three CD8⁺T cell epitopes is fused to the C-terminal of the GLBP protein. The underlined amino acid sequences of WT1 protein were transduced into B. longum 420. SP ^R: spectinomycin resistance gene. The expression of GLBP-WT1 fusion protein on B. longum 420 was detected by b Western blotting analysis and c immunofluorescence staining