Fig. 2.

Functional evaluation of anti-hDEC205 and anti-hDEC205-WT1_small antibody fusion proteins. a FACS analyses to assess binding capacities of the antibody fusion proteins to human DEC205. Top CHO-hDEC205 cells. Bottom parental CHO cells. Humira, anti-hTNF-α antibody served as a negative control. An internal identity of the anti-hDEC205-WT1_91–138, -WT1_223–273, and -WT1_324–371 antibody fusion proteins: aDEC-P3, -P4, and -P5. b Specific binding of anti-hDEC205 antibody to the DEC205 on human immature moDCs detected by FACS analysis. c Equilibrium binding studies confirming the binding capability of the anti-hDEC205-WT1_small antibody fusion proteins to hDEC205. total binding, specific binding, nonspecific binding of the scFv:hDEC205-GpL by blocking with the parental antibody or the respective anti-hDEC205-WT1_small fusion proteins: anti-hDEC205 antibody (i), anti-hDEC205-WT1_91–138 (ii), anti-hDEC205-WT1_223–273 (iii), anti-hDEC205-WT1_324–371 (iv). RLU relative light unit, pM picomolar