Fig. 3.

DC-mediated T-cell response to anti-hDEC205-WT1_small antibody fusion proteins. a Compiled data from all individuals (n = 16) analyzed for IFN-γ⁺ T-cell responses by ICS. Shown are median frequencies with interquartile range (IQR), one-way ANOVA, Tukey’s test **p < 0.01. b IFN-γ ICS and FACS analysis of cells from one patient (PN2). Top CD3⁺IFN-γ⁺ T-cell frequencies gated on lymphocytes, middle and bottom frequencies of CD3⁺-gated CD4⁺IFN-γ⁺ and CD8⁺IFN-γ⁺ T-cells. T-cells were co-incubated with unloaded moDCs (unstimul.), with moDCs loaded with anti-hDEC205 antibody, anti-hDEC205-WT1_91–138 antibody fusion protein, WT1 peptide pool, or CMV pp65 protein