Fig. 5.

IFN-γ response of whole PBMCs from two AML patients after allo-HSCT assessed by IFN-γ ELISPOT assay. a Triplicate of a representative IFN-γ ELISPOT assay. b Summary analysis of the IFN-γ ELISPOT assay of two patients. Shown are means with SEM, unpaired Student t test ***p < 0.001. c Cytotoxic effector function of in vitro expanded WT1-specific T-cells induced by anti-hDEC205-WT1_91–138-loaded mature moDCs. Each VITAL-FarRed cytotoxicity experiment was performed in duplicates and is shown by means with SEM from three different patients (from top PN 2, 4, 3). d Cytotoxicity of T-cells ex vivo by IFN-γ ICS combined with CD107a degranulation assay. unstimulated, anti-hDEC205 Ab, anti-hDEC205-WT1_91–138, WT1 peptide pool stimulation. Shown are mean ± SD from five patients including PN 2, 3, 4. Tukey’s test **p < 0.01; ***p < 0.001