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Evaluation of interferon-γ (IFN-γ)-secreting T cell populations after in vitro re-stimulation with peptide pool or PBS. At the end of the protocol combining the multi-peptide vaccine with weekly metronomic chemotherapy in C57BL/6 mice, single-cell splenocyte suspension cultures were obtained and re-stimulated ex vivo for 5 h. ICS for IFN-γ was evaluated in CD4+ and CD8+ T cell populations
