Fig. 7.

Reduced suppressive capacity of intratumoral myeloid cells following Salmonella treatment. a CD11b⁺ cells were isolated from the spleens of normal or Salmonella-infected mice and added at a final concentration of 10 % to cultures of CD4⁺ T cells. Alternatively, CD11b⁺ cells were isolated from the spleens (b) or tumors (c) of tumor-bearing mice, with or without Salmonella treatment (“NT”: non-treated, “T”: treated), and added at a final concentration of 1 or 10 % to CD4 T cells. All cultures were set up in triplicates and IFN-γ production was assayed in supernatants collected after 24 h. The data are expressed as percentage suppression in IFN-γ production compared with cultures containing stimulated T cells alone. Asterisks denote statistically significant differences between the indicated groups (**p < 0.01; *p < 0.05)