Fig. 6.

The regulation of JAK2–STAT3 signaling pathway is involved in H. pylori-induced cell proliferation and migration by activating the downstream target genes BCL-2 and TWIST1. a, b qRT-PCR results of BCL-2 and TWIST1 mRNA in BGC-823 (a) and GES-1 (b) cells after JAK2 silencing and H. pylori infection for 12 h; GAPDH was used as an internal control. The results were normalized to the mRNA level transfected with negative control of siRNAs. c, d Western blotting and quantitative analysis of BCL-2 and TWIST1 expression in BGC-823 (c) and GES-1 (d) cells after JAK2 silencing and H. pylori infection for 24 h; β-actin expression was used as an internal control. e, f CCK-8 results of BGC-823 (e) and GES-1 (f) cells after BCL-2 silencing and H. pylori infection for the indicated times. g, h The migratory ability of BGC-823 (g) and GES-1 (h) cells was evaluated by transwell assays after TWIST1 silencing and H. pylori infection for 12 h. Representative image fields of the migratory cells on the membrane stained with crystal violet (×200), and OD₅₇₀ of crystal violet stain was determined to quantify the transwell assays. The data are represented as the mean ± SD of triplicate samples, *P < 0.05, **P < 0.01 versus the control group