Fig. 1.

Characterization of genes differentially expressed in pre-treatment tumors between the CA and No-CA groups. The Ingenuity IPA tool was used to characterize the 194 differentially expressed probe sets. a Top biological functions clustering the differentially expressed genes. The P value indicates the significance of the enrichment of input genes in each functional category. b Top canonical pathways clustering the differentially expressed genes. The P value (blue bar) indicates the significance of the enrichment of input genes in each pathway. The ratio (orange square) is the number of genes from the input list that were annotated to the pathway to the total number of genes annotated to the pathway. c, d Heatmap of the expression of the top 26 potential predictive tumor biomarkers. The tumors are grouped by time, before (c) and after (d) treatment with ipilimumab, and by CA status. Color indicates expression level, from low (blue) to high (red)