Fig. 3.

Characterization of genes with decreased expression after ipilimumab treatment. The Ingenuity IPA tool was used to characterize the 269 probe sets with decreased expression after treatment with ipilimumab. a Top biological functions clustering the genes. The P value indicates the significance of the enrichment of input genes in each functional category. b Top canonical pathways clustering the genes. The P value (blue bar) indicates the significance of the enrichment of input genes in each pathway. The ratio (orange square) is the number of genes from the input list that were annotated to the pathway to the total number of genes annotated to the pathway. c–f Heatmap of selected genes with decreased expression in post-treatment tumors from patients of the CA group. Figure shows expression of melanoma-associated markers before (c) and after (d) the treatment, and genes associated with cell proliferation before (e) and after (f) the treatment. Color indicates expression level, from low (blue) to high (red)