Fig. 1.

Apoptosis of NK cells after treatment with H₂O₂. a Purified NK cells were incubated with H₂O₂ for 24 h at indicated doses within physiological levels, and then subjected to apoptosis analysis with 7-AAD staining in combination with CD56 mAb. We prepared resting NK, IL-2 NK (500 IU/mL of IL-2), and expanded NK from the same healthy donor. Representative flow cytometric data from the same healthy donor are shown. b Summarized data of the apoptosis analysis with 7-AAD staining in NK cells from different donors (n = 8) are shown. ***p < 0.001, **p < 0.01, *p < 0.05