Fig. 3.

Clonality and functional profile of TIL-derived T cells reactive to ΕΝΟ1. a T cell clones were tested for proliferation in response to ENO1 in the presence of irradiated autologous APCs. Results are expressed as mean values of mitogenic index obtained in triplicate microcultures. b TCR Vβ chain collection of ENO1-specific T cell clones. The clonality of T cell clones reactive to ENO1 was analyzed by a panel of 24 monoclonal antibodies specific for human TCR Vβ chain families. T cell blasts from each clone were divided into aliquots and stained with each monoclonal antibody and the appropriate isotype controls. c Functional profile distribution of CD4⁺ ENO1-specific T cell clones