Fig. 4.
Presence of anti p185neu Abs in the sera of young and old mice after vaccination with pCMV ECDTM plasmid. Sera from mice immunized with pCMV ECDTM plasmid by IM or IM + E or with pCMV were collected before TUBO cell challenge. Specific p185 Sbp was evaluated by flow cytometry after indirect immunofluorescence and calculated as reported in Mat and Methods. Data shown are representative of one of the three independent experiments. *P at least <0.05 versus HER-2/neu IM old value; **P at least <0.05 versus respective young value; § P < 0.05 versus HER-2/neu IM young value