Fig. 2.
MHC class II restriction of MAGE-A3 and -A4 specific CD4+ T clones. a Example of class II restricted allele assessment on LAU 2069 CD4+ T-cell clone 1 specific for MAGE-A4291–305. Where indicated, anti-DP, DQ, DR purified antibodies were pre-incubated with cells prior to peptide stimulation. IFN-γ release was measured in the supernatant by ELISA. b Example of fine HLA II restriction performed on LAU 2069 clone 1. HLA class II matched LCL cell lines were pulsed (black bars) or not (white bars) with MAGE-A4291–305 peptide and presented to the specific clone. Recognition of the LCL cells with the restricting allele was measured by ELISA IFN-γ. c Summary table of MHC class II restriction of the cloned responses