Fig. 4.

Natural processing and presentation of identified MAGE-A3 and -A4 peptides. a Mature HLA-matched dendritic cells were activated with LPS and pulsed with 5 μM of recombinant MAGE-A3 protein. After washing, DCs were cocultured with CD4⁺ T-cell clones at 1:1 ratio. IFN-γ production was measured after 20 h in the supernatant by ELISA. Shown are example of MAGE-A3 161–175 peptide processing by HLA-DRB1*07 mature DC and presentation to LAU 2068 clone 4. b Recognition of the endogenous MAGE-A3 antigen was assessed upon HLA-matched EBV cells transduction with a retroviral construct encoding Ii.MAGE-A3 and coculture with MAGE-A3 peptide-specific clones. Shown is example of LAU 2091 clone 1 specific for epitope 111–125 cocultured with HLA-matched RULE LCL transduced with MAGE-A3. c Summary table of the described epitopes and natural processing