Fig. 5.
Naive T-cell priming against MAGE-A3 antigens depends on the mode of antigen delivery. Naive T cells were stimulated with immature DCs loaded with rhMAGE-A3 protein, opsonized rhMAGE-A3 antibody (6C1 IgG2a), rhMAGE-A3-immune complex and MAGE-A3-expressing U266 cells, pretreated with bortezomib. After two stimulation rounds INF-γ secretion of primed T cells was detected by ELISPOT. As ELISPOT targets, DCs were loaded with rhMAGE-A3 protD-fusion-protein, control rhMAGE-A3 protein and a MAGE-A3 peptide mix containing immunogenic HLA class I and class II epitopes, respectively. IFN-γ response of T cells is calculated by the mean of the DC/T-cells-derived spots subtracted by background spots. Shown are mean ± SEM of 4 independent experiments, 1-way ANOVA with Bonferroni’s multiple comparison test, *p < 0.05, **p < 0.01, ***p < 0.001