Fig. 5.
Expression of chemokines and corresponding chemokine receptors by Treg in unaffected small intestine (SI) and adenomas. The expression of CCL17 (a), CCL20 (b), CCL25 (c) and CXCL11 (d) mRNA was examined in WT and APCMin/+ SI tissue and adenomas from 18-week-old mice by real-time PCR and normalized against β-actin. The frequencies of CCR4+ (a), CCR6+ (b), CCR9+ (c) and CXCR3+ (d) cells among CD4+FoxP3+ cells were analyzed by flow cytometry in WT small intestinal lamina propria (LP) and APCMin/+ LP and adenomas from 18-week-old mice. Symbols represent individual mice, and lines represent median. Mann–Whitney test was used as statistical analysis *p < 0.05; **p < 0.01