Fig. 6.

Cytokine producing intestinal T cells in unaffected intestinal tissue and adenomas. Lymphocytes were isolated from intestinal lamina propria (LP) from both WT and APC^Min/+ mice and from adenoma tissue. The frequencies of IL-17A⁺ (a) and IFN-γ⁺ cells (b) among CD4⁺ or CD8⁺ T cells were determined by flow cytometry following PMA/ionomycin stimulation. IL-17A and IFN-γ mRNA expression (c) was determined in small intestine from both WT and APC^Min/+ mice, by real-time PCR and normalized against β-actin. Symbol represents individual mice, and bar represents median